AQUA® software
AQUAnalysis™ Software
Images acquired with AQUAsition software are used to localize and quantify various protein biomarker concentrations with unparalleled accuracy and reproducibility using AQUAnalysis software. The AQUAnalysis software uses proprietary algorithms to identify and localize cellular and sub-cellular (e.g. nucleus, cytoplasm, membrane) compartments of protein biomarkers. Areas of interest are flagged for operator review.
The AQUAnalysis software’s algorithms and compartmentalization combine to provide an AQUA score reflecting protein concentration in a molecularly defined area—true biomarker localization. Data is presented with significant data stratification, identifying subpopulations not seen with traditional IHC.
AQUAsition™ Software
Our digital imaging software allows the user to accurately identify and image individual tissue fields at multiple wavelengths in both tissue microarrays and whole tissue sections. Signal resolution rivals confocal microscopy while eliminating the visual subjectivity associated with conventional IHC.
AQUAServe™ software
AQUAServe™ software is a cloud-based server implementation of HistoRx’s flagship AQUAnalysis product. AQUAserve software uses AQUAnalysis proprietary algorithms operating on a remote server to identify and localize cellular and sub-cellular (e.g. nucleus, cytoplasm, membrane) compartments of protein biomarkers. The software’s algorithms and compartmentalization combine to provide an AQUA® score reflecting protein concentration in a molecularly defined area—true biomarker localization. Data is presented with significant data stratification, identifying subpopulations not seen with traditional IHC. Areas of interest are flagged for operator review with AQUAnalysis operating on a local workstation.
Instrumentation
Vectra® 2 from Caliper Life Sciences, a PerkinElmer Company
Vectra’s automated, high-throughput imaging capabilities make it a powerful tool for today’s preclinical and clinical studies. Combining three key components – automated slide-handling, multispectral imaging technology and pattern recognition-based image analysis – Vectra 2 is an advanced system for extracting proteomic and morphometric information from intact tissue sections. Vectra 2 is well suited for automated applications in:
- Tissue biomarker discovery and validation
- Signaling pathway analysis in cancer research and drug development
- Microenvironment analysis, including assessment of tumor immunogenicity
- Tissue cytometry, including multiplexed per-cell measures of nuclear, cytoplasmic, and membranous protein expression”
ScanScope® FL from Aperio
AQUA analysis is now available for use with fluorescent images acquired by the Aperio ScanScope FL. The partnership between HistoRx and Aperio was designed to meet the growing demand for complex analysis of multiple fluorescent biomarkers. This system integrates state of the art software and hardware to provide superior image quality and data analysis/integrity.
Features include:
- High Throughput
- Whole Slide imaging (providing context)
- Broad Range of Fluorescent applications
- Image analysis by biological design
- Database for storage/access of image results/data
- For use with whole tissues and tissue microarrays
- Provides continuous expression- Data analyzed on a continuous scale
PM-2000 from HistoRx
The PM -2000 is a dedicated tissue microarray reader and microscopy-based multiplex imaging device that rapidly analyzes tissue microarray samples and individual tissue slices, generating a quantitative score representing protein expression in the sample while retaining the spatial relationships.
Features Include:
- incorporates the AQUA® tissue analysis software
- reproducibly & rapidly measures protein concentrations within subcellular compartments
- uncompromising quantification capabilities with user-designed multiplexing
- uses standard histochemistry in tissue microarrays with up to 800 tissue samples per slide
- proprietary fluorescence-based imaging technology
- images stored in database for validation and archiving
Click here for more information on the Aperio /HistoRx partnership